The second edition of the Transylvanian Experimental Neuroscience Summer School

June 17th, 2013

The second edition of TENSS will take place between 1-17 July, 2013. The invited lecturers are:

  • Bhalla, Upinder – National Centre for Biological Sciences, Bangalore, India
  • Burrone, Juan – King’s College, London, UK
  • Emiliani, Valentina – Université Paris Decartes, Paris, France
  • Engert, Florian – Harvard University, Cambridge, MA, USA
  • Hofer, Sonja – University College London, London, UK
  • Hromádka, Tomáš – Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
  • Hübener, Mark – Max Planck Institute of Neurobiology, Martinsried, Germany
  • Kampff, Adam – Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
  • McNaughton, Bruce – Lethbridge University, Lethbridge, Canada
  • Mody, Istvan – Univeristy of California Los Angeles, Los Angeles, CA, USA
  • Monyer, Hannah – University of Heidelberg, Heidelberg, Germany
  • Mrsic-Flögel, Tom – University College London, London, UK
  • Murthy, Venkatesh – Harvard University, Cambridge, MA, USA
  • Nikolić, Danko – Max Planck Institute for Brain Research, Frankfurt, Germany
  • Oşan, Remus – Georgia State University, Atlanta, GA, USA
  • Rumpel, Simon – Institute for Molecular Pathology (IMP),Vienna, Austria
  • Singer, Wolf – Max Planck Institute for Brain Research, Frankfurt, Germany
  • Triesch, Jochen – Frankfurt Institute for Advanced Studies (FIAS), Frankfurt, Germany

The studied topics are:


  • Basic Optics.
  • Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
  • Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
  • Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
  • Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
  • Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb.
  • Scanning microscopy – Confocal and two-photon microscopy. Students will build a custom two-photon microscope.
  • Viral approaches to label, monitor and alter neuronal circuits.
  • Optogenetics – Light activated ion channels and pumps.
  • Basic electrophysiology – Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
  • Awake head fixed optical and electrophysiological recording strategies in rodents.
  • Synaptic plasticity – Long and short term plasticity. Homeostasis.
  • Techniques for electrophysiological data analysis.
  • Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
  • Synchrony and oscillations.
  • Cortical attention, sparse neuronal codes.
  • Decision making, uncertainty, neuro-modulatory systems.
  • Introduction to theoretical and computational modeling of neuronal circuits.

See the school’s website