The second edition of TENSS will take place between 1-17 July, 2013. The invited lecturers are:
- Bhalla, Upinder – National Centre for Biological Sciences, Bangalore, India
- Burrone, Juan – King’s College, London, UK
- Emiliani, Valentina – Université Paris Decartes, Paris, France
- Engert, Florian – Harvard University, Cambridge, MA, USA
- Hofer, Sonja – University College London, London, UK
- Hromádka, Tomáš – Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
- Hübener, Mark – Max Planck Institute of Neurobiology, Martinsried, Germany
- Kampff, Adam – Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
- McNaughton, Bruce – Lethbridge University, Lethbridge, Canada
- Mody, Istvan – Univeristy of California Los Angeles, Los Angeles, CA, USA
- Monyer, Hannah – University of Heidelberg, Heidelberg, Germany
- Mrsic-Flögel, Tom – University College London, London, UK
- Murthy, Venkatesh – Harvard University, Cambridge, MA, USA
- Nikolić, Danko – Max Planck Institute for Brain Research, Frankfurt, Germany
- Oşan, Remus – Georgia State University, Atlanta, GA, USA
- Rumpel, Simon – Institute for Molecular Pathology (IMP),Vienna, Austria
- Singer, Wolf – Max Planck Institute for Brain Research, Frankfurt, Germany
- Triesch, Jochen – Frankfurt Institute for Advanced Studies (FIAS), Frankfurt, Germany
The studied topics are:
Topics
- Basic Optics.
- Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
- Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
- Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
- Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
- Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb.
- Scanning microscopy – Confocal and two-photon microscopy. Students will build a custom two-photon microscope.
- Viral approaches to label, monitor and alter neuronal circuits.
- Optogenetics – Light activated ion channels and pumps.
- Basic electrophysiology – Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
- Awake head fixed optical and electrophysiological recording strategies in rodents.
- Synaptic plasticity – Long and short term plasticity. Homeostasis.
- Techniques for electrophysiological data analysis.
- Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
- Synchrony and oscillations.
- Cortical attention, sparse neuronal codes.
- Decision making, uncertainty, neuro-modulatory systems.
- Introduction to theoretical and computational modeling of neuronal circuits.