The third edition of the Transylvanian Experimental Neuroscience Summer School

January 8th, 2014

The third edition of TENSS will take place between 1-17 July, 2014. The confirmed lecturers are:

  • Florin Albeanu
  • Upinder Bhalla
  • Juan Burrone
  • Florian Engert
  • Viviana Gradinaru
  • Sonja Hofer
  • Tomáš Hromádka
  • Mark Hübener
  • Adam Kampff
  • Steffen Katzner
  • Georg Keller
  • Hannah Monyer
  • Tom Mrsic-Flögel
  • Raul Mureşan
  • Venkatesh Murthy
  • Danko Nikolić
  • Bence Ölveczky
  • Wolf Singer

The studied topics are:


  • Basic Optics.
  • Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
  • Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
  • Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
  • Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
  • Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb.
  • Scanning microscopy – Confocal and two-photon microscopy. Students will build a custom two-photon microscope.
  • Viral approaches to label, monitor and alter neuronal circuits.
  • Optogenetics – Light activated ion channels and pumps.
  • Basic electrophysiology – Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
  • Awake head fixed optical and electrophysiological recording strategies in rodents.
  • Synaptic plasticity – Long and short term plasticity. Homeostasis.
  • Techniques for electrophysiological data analysis.
  • Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
  • Synchrony and oscillations.
  • Cortical attention, sparse neuronal codes.
  • Decision making, uncertainty, neuro-modulatory systems.
  • Introduction to theoretical and computational modeling of neuronal circuits.

See the school’s website